Edvotek instructional videos – Diluting a

concentrated solution. Dilution is a technique used to decrease the

concentration of a solution by adding water or another solvent. For this

technique you will need: a concentrated solution distilled water and graduated cylinders. Step 1: Determine the dilution factor. Let’s say you need to prepare one

liter of diluted or 1x TAE buffer for your electrophoresis experiments. However, your Edvotek kit contains TAE buffer that is 50X, which is 50 times

more concentrated than you need. To get the working concentration of 1 X you will dilute the initial stock 1 to 50. Step 2: determine the amount of solvent

needed using the simple dilution formula C1 times V1 equals C2 times V2 where “C1” is the initial concentration,

“V1” is the initial volume, and “C2” and “V2” are the

final concentration and volume, respectively. In this equation we will simply plug in the known quantities and solve. In this example the initial

concentration you are working with is 50x. The final concentration of the

solution is 1x, and the final volume will be one liter, or 1,000 milliliters. By solving for V 1 we have calculated that 20 milliliters of 50x TAE are

needed to prepare 1 liter or 1,000 milliliters of 1x TAE. Step 3: Perform the dilution To make 1000 mL of 1x TAE begin by adding 20 mL of the 50x TAE to a large beaker. Next, measure and add 980

milliliters of distilled water to the beaker after you add the water the final

volume will be one liter. Mix thoroughly before using.